简 历

1999年在北京大学获得理学博士学位；

2000-2002年在日本日立中央研究所从事博士后研究；

2002-2013年河北大学教授、博士生导师；2005年获得教育部“新世纪优秀人才支持计划”资助；2007年获得“全国优秀教师”称号；2009年获得“国家杰出青年科学基金”资助；2010年获得河北省自然科学一等奖； 2012年所指导的博士论文获得“全国百篇优秀博士学位论文”提名；2013年获得国家自然科学基金重点项目资助；

2013-2016年陕西师范大学教授、博士生导师；

2016年至今北京科技大学教授、博士生导师；2016年入选国务院政府特殊津贴专家。

科研业绩

基于核酸扩增及纳米粒子探针信号放大技术，系统研究建立了基因突变、DNA甲基化、miRNA及mRNA的高灵敏度分析方法；基于单微球表面成像实现了生物标志的单分子、单细胞分析。先后主持国家杰出青年基金，国家自然科学基金重点项目及可控自组装及其功能化重大研究计划等20余项科研项目。在JACS, Angew. Chem. Int. Ed., Chem. Sci., Chem. Commun., Anal., Chem.等杂志发表论文120余篇。

获得奖励/专利

李正平(1/5); 利用分子发光技术研究高灵敏度分子诊断分析, 河北省人民政府,自然科学, 省部一等奖, 2010；

李正平(1/5); 新型生物标志物microRNA的高灵敏度、高选择性分析研究, 河北省人民政府, 自然科学, 省部二等奖, 2018；

李正平(2/6); 高灵敏度分子诊断新原理新方法研究，陕西高等学校科学技术奖一等奖，2020；

专利： 李正平; 王洪红; 王辉; 刘成辉; 一种茎环结构组合探针及其应用, 2018-3-6, 中国, ZL201610056612.1；

李正平; 王辉; 王洪红; 孙圆圆; 一种基于连接反应构建双茎环结构DNA模板检测核酸的方法, 2018-05-08, 中国, ZL201610316771.0；

李正平; 严景丽; 刘伟亮; T3 DNA连接酶和T4 RNA连接酶2在检测N6甲基腺嘌呤中的应用, 2019-08-20, 中国, ZL201611020857.5；

王辉; 李正平; 王洪红; 孙茹艳; 贾玉婷, 一种基于智能设备辅助的核酸定量分析方法及其应用, 201910538333.2, 中国；

Hui Wang, Zhengping Li, Honghong Wang, Ruyan Sun, Yuting Jia, Smart Equipment-Assisted Quantitative Analysis for Nucleic Acids and Use of the Same, PCT/CN2020/091831, PCT.

招生计划

每年博士生2-3名，硕士生3-4名。

代表性论文著作

[1] Mai Zhang, Honghong Wang, Hui Wang,* Fangfang Wang, and Zhengping Li*, CRISPR/Cas12a-Assisted Ligation-Initiated Loop-Mediated Isothermal Amplification (CAL-LAMP) for Highly Specific Detection of microRNAs, Analitical Chemistry 2021, 93, 7942−7948.

[2] Kejian Gao, Pengbo Zhang, Hui Wang, Honghong Wang, Fengxia Su, and Zhengping Li *, Ultrasensitive homogeneous detection of microRNAs in a single cell with specifically designed exponential amplification, Chemical Communications., 2021, 57, 5570–5573.

[3] Fangfang Wang, Yi Zhang, Desheng Chen, Zhuoqi Zhang,*, Zhengping Li,* Ultrasensitive detection of tumor-specific exosomal proteins by a Single Microbead-based Aptasensor coupled with Terminal deoxynucleotidyl transferase-initiated DNA amplification (SMAT), Sensors & Actuators: B. Chemical, 2021, 341, 130034.

[4] FangfangWang , HuiWang , Pengbo Zhang , Fengxia Su , HonghongWang , Zhengping Li *, Ultrasensitive multiplexed detection of miRNA targets of interest based on encoding probe extension in improved cDNA library, Analytica Chimica Acta 1152 (2021) 338281.

[5] Fangfang Wang, Yi Zhang, Desheng Chen, Zhuoqi Zhang* and Zhengping Li *, Single microbead-based fluorescent aptasensor (SMFA) for direct isolation and in situ quantification of exosomes from plasma, Analyst, 2021, 146, 3346-3351.

[6] Hui Wang, Honghong Wang, Yansheng Li, Chao Jiang, Desheng Chen, Yongqiang Wen*, ZhengpingLi*, Capillarity self-driven DNA hydrogel sensor for visual quantification of microRNA, Sensors and Actuators B: Chemical, 2020, 313, 128036.
[7] Hui Wang, Honghong Wang, Yuanyuan Sun, Xiaoling Liu, Ye Liu, Chengli Wang, Pengbo Zhang, Zhengping Li*, A general strategy for highly sensitive analysis of genetic biomarkers at single-base resolution with ligase-based isothermally exponential amplification, Talanta, 2020, 212: 120754.

[8] Pengbo Zhang, Na Guo, Kejian Gao, Fengxia Su, Fangfang Wang and Zhengping Li*, Direct recognition and sensitive detection of circular RNA with ligation-based PCR, Org. Biomol. Chem., 2020,18, 3269-3273.

[9] Yiqian Ren, Yao Qiang, Xinrui Duan* and Zhengping Li*, The distinct difference in azido sugar metabolic rate between neural stem cells and fibroblasts and its application for decontamination of chemically induced neural stem cells, Chem. Commun., 2020,56, 2344-2347.

[10] Zhenhao Zhang, Jingli Yan*, and Zhengping Li*, Peroxotungstate oxidation-mediated two-phase amplification system (POM-TPAS) for bisulfite-free quantification of locus-specific 5-hydroxymethylcytosine, Chem. Commun., 2020, 56, 3111-3114.

[11] Zhenhao Zhang; Xiao Shan; Pengbo Zhang; Weiliang Liu; Jingli Yan(*); Zhengping Li(*)，Highly sensitive quantification of site-specific 5-hydroxymethylcytosine at single-base resolution by HpaII-mediated ligation PCR， Organic & Biomolecular Chemistry, 2019.11.13, 17: 9849-9853.

[12] Honghong Wang; Hui Wang(*); Yuting Jia; Mai Zhang; Zhengping Li(*)，One-pot detection of telomerase activity with high sensitivity and specificity via RNA FRET probes and RNase H-assisted signal cycling amplification， RSC Advances, 2019.4.28, 9: 14817-14821.

[13] Honghong Wang; Hui Wang; Mai Zhang; Yuting Jia; Zhengping Li(*)，A label-free aptamer-based biosensor for microRNA detection by the RNA-regulated fluorescence of malachite green， RSC Advances, 2019.10.2, 9: 32906-32910.

[14] Hui Wang; Honghong Wang; Yuting Jia; Ruyan Sun; Weixiang Hong; Mai Zhang; Zhengping Li*, Visual Detection of Fusion Genes by Ligation-Triggered Isothermal Exponential Amplification: A Point-of-Care Testing Method for Highly Specific and Sensitive Quantitation of Fusion Genes with a Smartphone, Analytical Chemistry, 2019, 91, 12428-42434.

[15] Weiliang Liu; Jingli Yan*; Zhenhao Zhang; Hongru Pian; Chenghui Liu; Zhengping Li*, Identification of a selective DNA ligase for accurate recognition and ultrasensitive quantification of N6-methyladenosine in RNA at one-nucleotide resolution, Chemical Science, 2018, 9, 3354-3359.

[16]Yuanyuan Sun; Hui Tian; Chenghui Liu*; Dandan Yang; Zhengping Li*, A Clamp-Based One-Step Droplet Digital Reverse Transcription PCR (ddRT-PCR) for Precise Quantitation of Messenger RNA Mutation in Single Cells, ACS Sensors, 2018, 3, 1795-1801.

[17] Yan Qi; Xiaohui Lu; Qinya Feng; Wenjiao Fan; Chenghui Liu*; Zhengping Li*, An Enzyme-Free MicroRNA Assay Based On Fluorescence Counting of Click Chemical Ligation-Illuminated Magnetic Nanoparticles with Total Internal Reflection Fluorescence Microscopy, ACS Sensors, 2018, 3, 2667-2674.

[18] Weimin Tian; Pengjie Li; Wenli He; Chenghui Liu*; Zhengping Li*, Rolling circle extension-actuated loop-mediated isothermal amplification (RCA-LAMP) for ultrasensitive detection of microRNAs, Biosensors and Bioelectronics, 2018, 128, 17-22.

[19] Yueying Sun; Yuanyuan Sun; Weimin Tian; Chenghui Liu*; Kejian Gao; Zhengping Li*, A novel restriction endonuclease GlaI for rapid and highly sensitive detection of DNA methylation coupled with isothermal exponential amplification reaction, Chemical Science, 2018, 9, 1344-1351.

[20] Hui Wang, Honghong Wang, Xinrui Duan*, Chenghui Liu, Zhengping Li*，Digital quantitative analysis of microRNA in single cell based on ligationdepended polymerase colony (Polony)，Biosensors and Bioelectronics 2017, 95, 146–151.

[21] Hui Wang, Honghong Wang, Xinrui Duan∗∗, Xiangdong Wang, Yuanyuan Sun,Zhengping Li∗，Sensitive detection of mRNA by using specific cleavage-mediated isothermal exponential amplification reaction，Sensors and Actuators B ，2017, 252, 215–221.

[22] Yuanyuan Sun, Hui Tian, Chenghui Liu, * Yueying Sun and Zhengping Li *，One-step detection of microRNA with high sensitivity and specificity via target-triggered loop-mediated isothermal amplification (TT-LAMP)，Chem. Commun., 2017, 53,11040-11043

[23] Xiangdong Wang, Hui Wang, Chenghui Liu,* Honghong Wang and Zhengping Li*，A three-way junction structure-based isothermal exponential amplification strategy for sensitive detection of 30-terminal 20-O-methylated plant microRNA，Chem. Commun., 2017, 53, 1124-1127.

[24] Liying Qiu, Yuecheng Zhang, Chenghui Liu* and Zhengping Li*, A versatile size-coded flow cytometric bead assay for simultaneous detection of multiple microRNAs coupled with a two-step cascading signal amplification, Chem. Commun., 2017, 53, 2926-2929.

[25] Honghong Wang, Hui Wang, Xinrui Duan,* Yuanyuan Sun, Xiangdong Wang and Zhengping Li*, Highly Sensitive and Multiplexed Quantification of mRNA Splice Variants by Direct Ligation of DNA Probes at Exon Junction and Universal PCR Amplification, Chemical Science, 2017, 8, 3635–3640.

[26] Desheng Chen, Xiaobo Zhang, Liping Zhu, Chenghui Liu,* and Zhengping Li*, Single Microbead-Anchored Fluorescent Immunoassay (SMFIA): A Facile and Versatile Platform Allowing Simultaneous Detection of Multiple Antigens, Chem. Asian J. 2017, 12, 2894 – 2898.

[27] Hui Tian, Yuanyuan Sun, Chenghui Liu,* Xinrui Duan, Wei Tang, and Zhengping Li*，Precise Quantitation of MicroRNA in a Single Cell with Droplet Digital PCR Based on Ligation Reaction，Anal. Chem. 2016, 88, 11384−11389.

[28] Hui Wang, Honghong Wang, Chenghui Liu, Xinrui Duan & Zhengping Li*, Enzyme-free and multiplexed microRNA detection using microRNA-initiated DNA molecular motor, Sci China Chem, 2016, 59: 83–88.

[29] Lingbo Sun, Hongxia Fu, Yanru Li, Xinrui Duan,* and Zhengping Li*, Rapid Recognition and Isolation of Live Colon Cancer Stem Cells by Using Metabolic Labeling of Azido Sugar and Magnetic Beads, Anal. Chem. 2016, 88, 3953−3958.

[30] Honghong Wang, Hui Wang, Chenghui Liu,* Xinrui Duan and Zhengping Li*, Ultrasensitive detection of telomerase activity in a single cell using stem-loop primer-mediated exponential amplification (SPEA) with near zero nonspecific signal, Chemical Science, 2016, 7, 4945–4950.

[31] Hui Wen, Hui Wang, Honghong Wang, Jingli Yan,* Hui Tian and Zhengping Li*，Ultrasensitive detection of site-specific DNA methylation by loop-mediated isothermal amplification，Anal. Methods, 2016, 8, 5372-5377.

[32] Wei Tang, Ting Zhang, Qinggui Li,* Hui Wang,a Honghong Wang and Zhengping Li*，Sensitive detection of tumor cells based on aptamer recognition and isothermal exponential amplification，RSC Adv., 2016, 6, 89888-94.

[33] Xiaobo Zhang, Chenghui Liu,* Lingbo Sun, Xinrui Duan and Zhengping Li*, Lab on a single microbead: an ultrasensitive detection strategy enabling microRNA analysis at the single-molecule level, Chemical Science, 2015, 6, 6213-6218.

[34] Xiaobo Zhang, Chenghui Liu,* Honghong Wang, Hui Wang, Zhengping Li*, Highly-Concentrated Fluorescence Enrichment on a Single Microbead: An Ultrasensitive Strategy for the Detection of Protein Kinase Activity at Single-Cell Level. Angewandte Chemie International Edition, 2015, 54, 15186-15190.

[35] Fengxia Su, Limei Wang, Yueying Sun, Chenghui Liu, Xinrui Duan and Zhengping Li*， Highly sensitive and multiplexed analysis of CpG methylation at single-base resolution with ligation based exponential amplification，Chemical Science，2015, 2015, 6, 1866-1872.

[36] Fengxia Su, Limei Wang, Yueying Sun, Chenghui Liu, Xinrui Duan, and Zhengping Li*, Highly sensitive detection of CpG methylation in genomic DNA by AuNP-based colorimetric assay with ligase chain reaction, Chem. Commun., 2015, 51, 3371-3374.

[37] Yuecheng Zhang, Chenghui Liu,* Sujuan Sun, Yanli Tang and Zhengping Li*, Phosphorylation-induced hybridization chain reaction on beads: an ultrasensitive flow cytometric assay for the detection of T4 polynucleotide kinase activity, Chem. Commun., 2015, 51, 5832-5835.

[38] Haoxian Zhou, Hui Wang, Chenghui Liu,* Honghong Wang, Xinrui Duan and Zhengping Li*, Ultrasensitive genotyping with target-specifically generated circular DNA templates and RNA FRET probes, Chem. Commun., 2015, 11556-11559.

[39] Yueying Sun, Xiaohui Lu, Fengxia Su, Limei Wang, Chenghui Liu, Xinrui Duan, Zhengping Li*, Real-time fluorescence ligase chain reaction for sensitive detection of single nucleotide polymorphism based on fluorescence resonance energy transfer. Biosens. Bioelectron. 2015, 74, 705-710.

研究生培养情况

先后招收博士后4名，博士生15名，已毕业博士生8名，全部在高等院校任教。招收硕士生50余名，毕业近40名，就业方向主要是继续攻读博士学位、出国进修、政府部门、高等院校、企业，部分学生获“科技新星”和国家优秀青年基金资助。